Anti-ABCB1 Polyclonal Antibody 2 is a Rabbit antibody targeting ABCB1. Anti-ABCB1 Polyclonal Antibody 2 can be used in FCM, ICC/IF, WB.

IgG

Human,Rat (predicted:Mouse)

1. Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti-MDR1 antibody (TMAB-00022)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 70% methanol (Overnight at-20°C) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Sample: liver (Rat) Lysate at 40 μg
Primary: Anti-MDR1/P Glycoprotein (TMAB-00022) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 141 kDa
Observed band size: 141 kDa
3. Tissue/cell: SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (MDR1/P Glycoprotein) polyclonal Antibody, Unconjugated (TMAB-00022) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
4. Tissue/cell: SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (MDR1/P Glycoprotein) polyclonal Antibody, Unconjugated (TMAB-00022) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

FCM=1 μg/Test; ICC/IF=1:100-500; WB=1:500-2000

Polyclonal

KLH conjugated synthetic peptide: human MDR1

Human

ABCB1

ATP-dependent translocase ABCB1

P glycoproteins,ATPases,Channels,Side Population,Surface Molecules

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Subunit : Interacts with PSMB5.