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Anti-14-3-3 epsilon Antibody (5E440)

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Catalog No. TMAC-00003
Alias tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, ε, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon, MDS, MDCR, KCIP-1, HEL2, 14-3-3E, 14-3-3 ε

Anti-14-3-3 epsilon Antibody (5E440) is a Rabbit antibody targeting 14-3-3 epsilon. Anti-14-3-3 epsilon Antibody (5E440) can be used in WB,ICC/IF,IHC,FCM.

Anti-14-3-3 epsilon Antibody (5E440)

Anti-14-3-3 epsilon Antibody (5E440)

😃Good
Catalog No. TMAC-00003Alias tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, ε, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon, MDS, MDCR, KCIP-1, HEL2, 14-3-3E, 14-3-3 ε
Anti-14-3-3 epsilon Antibody (5E440) is a Rabbit antibody targeting 14-3-3 epsilon. Anti-14-3-3 epsilon Antibody (5E440) can be used in WB,ICC/IF,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2977-10 days
100 μL$4967-10 days
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Product Introduction

Bioactivity
Description
Anti-14-3-3 epsilon Antibody (5E440) is a Rabbit antibody targeting 14-3-3 epsilon. Anti-14-3-3 epsilon Antibody (5E440) can be used in WB,ICC/IF,IHC,FCM.
Synonymstyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, ε, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon, MDS, MDCR, KCIP-1, HEL2, 14-3-3E, 14-3-3 ε
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of 14-3-3 epsilon on different lysates using anti-14-3-3 epsilon antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y, Lane 2: 293T.
2. Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-14-3-3 epsilon antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-14-3-3 epsilon antibody. Counter stained with hematoxylin.
4. ICC staining 14-3-3 epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining 14-3-3 epsilon in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining 14-3-3 epsilon in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 epsilon antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:10-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThe 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, ??, ζ, ?? and η that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 29 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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