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Anti-14-3-3 beta Antibody-PerCP (9I180)
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Catalog No. TMAY-01251C
Alias tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, 14-3-3 β
Anti-14-3-3 beta Antibody-PerCP (9I180) is a PerCP-conjugated Mouse antibody targeting 14-3-3 beta. Anti-14-3-3 beta Antibody-PerCP (9I180) can be used in FCM.
Anti-14-3-3 beta Antibody-PerCP (9I180)
Anti-14-3-3 beta Antibody-PerCP (9I180)
😃Good
Catalog No. TMAY-01251CAlias tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, 14-3-3 β
Anti-14-3-3 beta Antibody-PerCP (9I180) is a PerCP-conjugated Mouse antibody targeting 14-3-3 beta. Anti-14-3-3 beta Antibody-PerCP (9I180) can be used in FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 T | $127 | 7-10 days | |
| 100 T | $258 | 7-10 days |
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Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-14-3-3 beta Antibody-PerCP (9I180) is a PerCP-conjugated Mouse antibody targeting 14-3-3 beta. Anti-14-3-3 beta Antibody-PerCP (9I180) can be used in FCM. |
| Alias | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, 14-3-3 β |
| Ig Type | Monoclonal Mouse IgG2b |
| Clone | 9I180 |
| Reactivity | Human |
| Verified Activity | Flow cytometric analysis of human YWHAB expression on Molt-4 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and then stained with PerCP-conjugated anti-human YWHAB. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM |
| Recommended Dose | 10 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD9 (rh CD9; TMPY-01266; NP_003395.1; Met1-Asn246) and conjugated with PerCP under optimum conditions, the unreacted PerCP was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.09% sodium azide |
| Research Background | 14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation. |
| Conjucates | PerCP |
| Others Formats | Unconjugated/APC/FITC/PE |
| Antibody Types Available | 5 |
| Immunogen | Recombinant Human 14-3-3 beta / YWHAB protein (TMPY-01266) |
| Antigen Species | Human |
| Biology Area | phospho-Serine/phospho-Threonine Binding Proteins, Adaptor Proteins, Apoptosis Adaptor Proteins |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| Transport | Shipping with blue ice. |
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