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Vitronectin Protein, Human, Recombinant (His)

(Synonyms: VNT, VN, vitronectin, V75) Copy Product Info

Synonyms: VNT, VN, vitronectin, V75

Catalog No. TMPY-00121 Copy Product Info
Vitronectin Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 53.7 kDa and the accession number is D9ZGG2.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$37-In Stock
10 μg$56-In Stock
20 μg$88-In Stock
50 μg$167In StockIn Stock
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:≥95 % as determined by SDS-PAGE.
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to Vitronectin-coated plates (10 μg/mL and 100 μL/well), approximately >40% cells will adhere specifically after 30 minutes at 37℃.
Description
Vitronectin Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 53.7 kDa and the accession number is D9ZGG2.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberD9ZGG2
ConstructionA DNA sequence encoding the human VTN (NP_000629.3) (Met1-Leu478) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Asp 20
Protein Purity
≥95 % as determined by SDS-PAGE.
Endotoxin< 10 EU/mg of the protein.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsVNT, VN, vitronectin, V75
Research Background
Chemical Properties
Molecular Weight53.7 kDa (predicted); 68.1 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
StorageStore at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: Vitronectin Protein, Human, Recombinant (His) chemical structure | Vitronectin Protein, Human, Recombinant (His) molecular weight