TsaE Protein, E. coli, Recombinant

Name: TsaE Protein, E. coli, Recombinant
Brand: TargetMol
SKU: TMPH-00745
Rating: 4.5 (1 reviews)

(Synonyms: yjeE, tsaE, tRNA threonylcarbamoyladenosine biosynthesis protein TsaE, t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE) Copy Product Info

Synonyms: yjeE, tsaE, tRNA threonylcarbamoyladenosine biosynthesis protein TsaE, t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE

Catalog No. TMPH-00745 Copy Product Info

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.

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Pack Size	Price	USA Stock	Global Stock
5 µg	$185	20 days	20 days
10 µg	$297	20 days	20 days
20 µg	$515	20 days	20 days
50 µg	$713	20 days	20 days
100 µg	$916	20 days	20 days
200 µg	$1,260	20 days	20 days
500 µg	$1,930	20 days	20 days
1 mg	$2,690	20 days	20 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.
Species	E. coli
Expression System	E. coli
Tag	Tag Free
Accession Number	P0AF67
Amino Acid	MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTFSRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFANDAICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG
Construction	1-153 aa
Protein Purity	> 90% as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Tris-based buffer, 50% glycerol
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	yjeE, tsaE, tRNA threonylcarbamoyladenosine biosynthesis protein TsaE, t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE
Research Background	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.

Chemical Properties

Molecular Weight

16.9 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.