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tPA Protein, Human, Recombinant (hFc)
(Synonyms: T-PA, TPA, plasminogen activator, tissue) Copy Product InfoSynonyms: T-PA, TPA, plasminogen activator, tissue
Catalog No. TMPY-00937 Copy Product Info
tPA Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 57.5 kDa and the accession number is P00750-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $128 | 7-10 days | 7-10 days | |
| 10 μg | $213 | 7-10 days | 7-10 days | |
| 20 μg | $357 | - | In Stock |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 95 % as determined by SDS-PAGE
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| Description | tPA Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 57.5 kDa and the accession number is P00750-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | N-hFc |
| Accession Number | P00750-1 |
| Construction | A DNA sequence encoding the α chain (Tyr 37-Arg 310) of mature human tPA (NP_000921.1) was expressed with the fused Fc region of human IgG1 at the N-terminus. Predicted N terminal: Glu 20 |
| Protein Purity | > 95 % as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM NaAc, 150 mM NaCl, 5 mM CaCl2, 10% glycerol, PH 5. 5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with 50 mM NaOAc, 150 mM NaCl, 5 mM CaCl2, pH 5.5 to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | T-PA, TPA, plasminogen activator, tissue |
| Research Background |
| Molecular Weight | 57.5 kDa (predicted); 59 kDa (reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: tPA Protein, Human, Recombinant (hFc) chemical structure | tPA Protein, Human, Recombinant (hFc) molecular weight
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