TIMP-2 Protein, Human, Recombinant

Name: TIMP-2 Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-01868
Price: 129 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: TIMP metallopeptidase inhibitor 2, DDC8, CSC-21K) Copy Product Info

Synonyms: TIMP metallopeptidase inhibitor 2, DDC8, CSC-21K

Catalog No. TMPY-01868 Copy Product Info

TIMP-2 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 22 kDa and the accession number is A0A140VK57.

Pack Size	Price	USA Stock	Global Stock
5 µg	$129	-	In Stock
10 µg	$215	-	In Stock
20 µg	$357	7-10 days	7-10 days
50 µg	$589	7-10 days	7-10 days
100 µg	$987	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:> 96 % as determined by SDS-PAGE

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2. The IC50 value is < 4 nM.
Description	TIMP-2 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 22 kDa and the accession number is A0A140VK57.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P16035
Construction	A DNA sequence encoding the mature form of human TIMP2 (NP_003246.1) (Cys 27-Pro 220) was expressed and purified. Predicted N terminal: Met
Protein Purity	> 96 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	TIMP metallopeptidase inhibitor 2, DDC8, CSC-21K
Research Background	Tissue inhibitors of metalloproteinases (TIMP) family are natural inhibitors of the matrix metalloproteinases (MMPs), the zinc enzymes involved in extracellular matrix maintenance and remodeling. The TIMP family encompasses four members (TIMP1-4), and they inhibit most MMPs by forming non-covalent binary complex. TIMP2 is a 22 kDa non N-glycosylated protein expressed by a variety of cell types, and plays a unique role among TIMP family members owing to its functions to regulate cellular responses to growth factors. Findings establish an unexpected, MMP-independent mechanism for TIMP2 inhibition of endothelial cell proliferation in vitro and reveal an important component of the antiangiogenic effect of TIMP2 in vivo. TIMP-2 thus is critical to the maintenance of tissue homeostasis and is involved in the regulation of tumor microenvironment.

Chemical Properties

Molecular Weight

22 kDa (predicted); 20 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.