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TIM14 Protein, S. cerevisiae, Recombinant
(Synonyms: TIM14, Presequencetranslocated-associated motor subunit PAM18, PAM18, Mitochondrial import inner membrane translocase subunit TIM14) Copy Product InfoSynonyms: TIM14, Presequencetranslocated-associated motor subunit PAM18, PAM18, Mitochondrial import inner membrane translocase subunit TIM14
Catalog No. TMPJ-01087 Copy Product Info
Mitochondrial import inner membrane translocase subunit TIM14 (TIM14) is an essential component of the PAM complex. PAM complex is required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, TIM14 is required to stimulate activity of mtHSP70 (SSC1). TIM14 belongs to the DnaJ family, which has been involved in Hsp40/Hsp70 chaperone systems. As a mitochondrial chaperone, TIM14 functions as part of the TIM23 complex import motor to facilitate the import of nuclear-encoded proteins into the mitochondria. TIM14 also complexes with prohibitin complexes to regulate mitochondrial morphogenesis, and has been implicated in dilated cardiomyopathy with ataxia.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $112 | 35 days | 35 days | |
| 10 μg | $183 | 35 days | 35 days | |
| 20 μg | $292 | 35 days | 35 days | |
| 50 μg | $545 | 35 days | 35 days | |
| 100 μg | $792 | 35 days | 35 days | |
| 200 μg | $1,150 | 35 days | 35 days | |
| 500 μg | $1,900 | 35 days | 35 days | |
| 1 mg | $2,970 | 35 days | 35 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| Description | Mitochondrial import inner membrane translocase subunit TIM14 (TIM14) is an essential component of the PAM complex. PAM complex is required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, TIM14 is required to stimulate activity of mtHSP70 (SSC1). TIM14 belongs to the DnaJ family, which has been involved in Hsp40/Hsp70 chaperone systems. As a mitochondrial chaperone, TIM14 functions as part of the TIM23 complex import motor to facilitate the import of nuclear-encoded proteins into the mitochondria. TIM14 also complexes with prohibitin complexes to regulate mitochondrial morphogenesis, and has been implicated in dilated cardiomyopathy with ataxia. |
| Species | S. cerevisiae |
| Expression System | E. coli |
| Tag | Tag Free |
| Accession Number | Q07914 |
| Amino Acid | Phe99-Lys168 |
| Construction | Phe99-Lys168 |
| Protein Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxin | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris-HCl, 300 mM NaCl, pH 8.0. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | TIM14, Presequencetranslocated-associated motor subunit PAM18, PAM18, Mitochondrial import inner membrane translocase subunit TIM14 |
| Research Background |
| Molecular Weight | 9 KDa (reducing condition) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: TIM14 Protein, S. cerevisiae, Recombinant chemical structure | TIM14 Protein, S. cerevisiae, Recombinant molecular weight
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