Thioredoxin/TRX Protein, Human, Recombinant

(Synonyms: TXN, TRX1, TRX, TRDX, thioredoxin, RP11-427L11.1, MGC61975, DKFZp686B1993) Copy Product Info

Synonyms: TXN, TRX1, TRX, TRDX, thioredoxin, RP11-427L11.1, MGC61975, DKFZp686B1993

Catalog No. TMPY-02250 Copy Product Info

Thioredoxin/TRX Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 11.7 kDa and the accession number is P10599.

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	-	In Stock
10 µg	$108	-	In Stock
20 µg	$178	-	In Stock
50 µg	$359	-	In Stock
100 µg	$696	-	In Stock
200 µg	$980	7-10 days	7-10 days
500 µg	$2,260	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:≥ 97 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to catalyze the reduction of insulin. The reaction leads toprecipitation, which can be measured by absorbance at 650 nm. The specific activity is 5-10 A650/min/mg.
Description	Thioredoxin/TRX Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 11.7 kDa and the accession number is P10599.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	P10599
Construction	A DNA sequence encoding the C-terminal segment of human TXN (P10599) (Met 1-Val 105) was expressed and purified. Predicted N terminal: Met
Protein Purity	> 97 % as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	TXN, TRX1, TRX, TRDX, thioredoxin, RP11-427L11.1, MGC61975, DKFZp686B1993
Research Background	Thioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein that belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Chemical Properties

Molecular Weight

11.7 kDa (predicted); 14 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.