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TFIIB Protein, Human, Recombinant (GST)

Catalog No. TMPJ-01067
Synonyms: TFIIB, GTF2B, TF2B, S300-II, General Transcription Factor TFIIB, Transcription Initiation Factor IIB

Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.

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TFIIB Protein, Human, Recombinant (GST)
Pack Size Availability Price/USD Quantity
10 μg 5 days $ 184.00
50 μg 5 days $ 545.00
500 μg 5 days $ 1,900.00
1 mg 5 days $ 2,730.00
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Biological Description
Technical Params
Product Properties
Description Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.
Species Human
Expression System E. coli
Tag N-GST
Accession Number Q00403
Synonyms TFIIB, GTF2B, TF2B, S300-II, General Transcription Factor TFIIB, Transcription Initiation Factor IIB
Amino Acid Met1-Leu316
Construction Recombinant Human Transcription Initiation Factor IIB is produced by our E.coli expression system and the target gene encoding Met1-Leu316 is expressed with a GST tag at the N-terminus.
Protein Purity Greater than 81% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight 62 KDa, reducing conditions
Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.0.
Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
Stability & Storage

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Research Background Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.

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Keywords

TFIIB Protein, Human, Recombinant (GST) TFIIB GTF2B TF2B S300-II General Transcription Factor TFIIB Transcription Initiation Factor IIB recombinant recombinant-proteins proteins protein

 

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