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Sphingosine Kinase 1/SPHK1 Protein, Human, Recombinant
(Synonyms: SPK, SPHK1, SPHK, sphingosine kinase 1) Copy Product InfoSynonyms: SPK, SPHK1, SPHK, sphingosine kinase 1
Catalog No. TMPY-04432 Copy Product Info
Sphingosine Kinase 1/SPHK1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 42.7 kDa and the accession number is Q9NYA1-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 10 µg | $152 | 7-10 days | 7-10 days | |
| 20 µg | $248 | 7-10 days | 7-10 days | |
| 50 µg | $577 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | The specific activity was determined to be ＞500nmol/min/mg using Sphingosine Kinase Substrate as substrate. |
| Description | Sphingosine Kinase 1/SPHK1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 42.7 kDa and the accession number is Q9NYA1-1. |
| Species | Human |
| Expression System | Baculovirus Insect Cells |
| Tag | Tag Free |
| Accession Number | Q9NYA1-1 |
| Construction | A DNA sequence encoding the human SPHK1 (Q9NYA1-1)(Met1-Leu384) was fused with two additional amino acids (Gly & Pro) at the N-terminus. Predicted N terminal: Gly |
| Protein Purity | > 85 % as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Supplied as sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Synonyms | SPK, SPHK1, SPHK, sphingosine kinase 1 |
| Research Background | SPHK1, also known as sphingosine Kinase 1, catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. |
| Molecular Weight | 42.7 kDa (predicted); 46 kDa (reducing conditions) |
| Shipping | Proteins are shipped with blue ice. |
| Storage | It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: Sphingosine Kinase 1/SPHK1 Protein, Human, Recombinant chemical structure | Sphingosine Kinase 1/SPHK1 Protein, Human, Recombinant molecular weight
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