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SFP Protein, B.subtilis, Recombinant (His)

Catalog No. TMPJ-00611
Synonyms: Surfactin synthase-activating enzyme, sfp, lpa-8, 4'-phosphopantetheinyl transferase sfp

The Bacillus subtilis enzyme Sfp, required for production of the lipoheptapeptide antibiotic surfactin, posttranslationally phosphopantetheinylates a serine residue in each of the seven peptidyl carrier protein domains of the first three subunits (SrfABC) of surfactin synthetase to yield docking sites for amino acid loading and peptide bond formation.

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SFP Protein, B.subtilis, Recombinant (His)
Pack Size Availability Price/USD Quantity
10 μg 5 days $ 146.00
50 μg 5 days $ 442.00
500 μg 5 days $ 2,060.00
1 mg 5 days $ 3,100.00
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Biological Description
Technical Params
Product Properties
Description The Bacillus subtilis enzyme Sfp, required for production of the lipoheptapeptide antibiotic surfactin, posttranslationally phosphopantetheinylates a serine residue in each of the seven peptidyl carrier protein domains of the first three subunits (SrfABC) of surfactin synthetase to yield docking sites for amino acid loading and peptide bond formation.
Species B.subtilis
Expression System E. coli
Tag C-6His
Accession Number CAA44858.1
Synonyms Surfactin synthase-activating enzyme, sfp, lpa-8, 4'-phosphopantetheinyl transferase sfp
Amino Acid Met1-Leu224
Construction Recombinant B.subtilis 4'-phosphopantetheinyl Transferase Sfp/Sfp is produced by our E.coli expression system and the target gene encoding Met1-Leu224 is expressed with a 6His tag at the C-terminus.
Protein Purity Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight 28 KDa, reducing conditions
Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation Supplied as a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, pH 7.0.
Stability & Storage

Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Shipping

The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Research Background The Bacillus subtilis enzyme Sfp, required for production of the lipoheptapeptide antibiotic surfactin, posttranslationally phosphopantetheinylates a serine residue in each of the seven peptidyl carrier protein domains of the first three subunits (SrfABC) of surfactin synthetase to yield docking sites for amino acid loading and peptide bond formation.

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Keywords

SFP Protein, B.subtilis, Recombinant (His) Surfactin synthase-activating enzyme sfp lpa-8 4'-phosphopantetheinyl transferase sfp recombinant recombinant-proteins proteins protein

 

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