The Bacillus subtilis enzyme Sfp, required for production of the lipoheptapeptide antibiotic surfactin, posttranslationally phosphopantetheinylates a serine residue in each of the seven peptidyl carrier protein domains of the first three subunits (SrfABC) of surfactin synthetase to yield docking sites for amino acid loading and peptide bond formation.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
10 μg | 5 days | $ 146.00 | |
50 μg | 5 days | $ 442.00 | |
500 μg | 5 days | $ 2,060.00 | |
1 mg | 5 days | $ 3,100.00 |
Description | The Bacillus subtilis enzyme Sfp, required for production of the lipoheptapeptide antibiotic surfactin, posttranslationally phosphopantetheinylates a serine residue in each of the seven peptidyl carrier protein domains of the first three subunits (SrfABC) of surfactin synthetase to yield docking sites for amino acid loading and peptide bond formation. |
Species | B.subtilis |
Expression System | E. coli |
Tag | C-6His |
Accession Number | CAA44858.1 |
Synonyms | Surfactin synthase-activating enzyme, sfp, lpa-8, 4'-phosphopantetheinyl transferase sfp |
Amino Acid | Met1-Leu224 |
Construction | Recombinant B.subtilis 4'-phosphopantetheinyl Transferase Sfp/Sfp is produced by our E.coli expression system and the target gene encoding Met1-Leu224 is expressed with a 6His tag at the C-terminus. |
Protein Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
Molecular Weight | 28 KDa, reducing conditions |
Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Supplied as a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, pH 7.0. |
Stability & Storage |
Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
Shipping |
The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
Research Background | The Bacillus subtilis enzyme Sfp, required for production of the lipoheptapeptide antibiotic surfactin, posttranslationally phosphopantetheinylates a serine residue in each of the seven peptidyl carrier protein domains of the first three subunits (SrfABC) of surfactin synthetase to yield docking sites for amino acid loading and peptide bond formation. |
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Please read the User Guide of Recombinant Proteins for more specific information.
SFP Protein, B.subtilis, Recombinant (His) Surfactin synthase-activating enzyme sfp lpa-8 4'-phosphopantetheinyl transferase sfp recombinant recombinant-proteins proteins protein