SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
10 μg | 5 days | $ 184.00 | |
50 μg | 5 days | $ 545.00 | |
500 μg | 5 days | $ 1,900.00 | |
1 mg | 5 days | $ 2,970.00 |
Description | SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone. |
Species | Rat |
Expression System | Human Cells |
Tag | C-6His |
Accession Number | P21581 |
Synonyms | steel factor, Mast cell growth factor, SLF, MGFSHEP7, SCFStem cell factor, SFc-Kit ligand, KL-1, KITLG, SCF, Kitl, MGF, KIT ligand, FPH2 |
Amino Acid | Gln26-Ala189 |
Construction | Recombinant Rat Kit Ligand is produced by our Mammalian expression system and the target gene encoding Gln26-Ala189 is expressed with a 6His tag at the C-terminus. |
Protein Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
Molecular Weight | 23-40 KDa, reducing conditions |
Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Stability & Storage |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
Shipping |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
Research Background | SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone. |
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Please read the User Guide of Recombinant Proteins for more specific information.
SCF Protein, Rat, Recombinant (HEK293, His) steel factor Mast cell growth factor SLF MGFSHEP7 FPH-2 SCFStem cell factor FPH 2 SFc-Kit ligand KL 1 KL-1 KITLG KL1 SCF MGFSHEP-7 Kitl MGF KIT ligand FPH2 MGFSHEP 7 recombinant recombinant-proteins proteins protein