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Synonyms: Runx1, Runt-related transcription factor 1, Polyomavirus enhancer-binding protein 2 alpha B subunit (PEA2-alpha B;PEBP2-alpha B), Oncogene AML-1, Core-binding factor subunit alpha-2 (CBF-alpha-2), Cbfa2, Aml1, Acute myeloid leukemia 1 protein


| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $176 | 20 days | 20 days | |
| 10 µg | $293 | 20 days | 20 days | |
| 20 µg | $491 | 20 days | 20 days | |
| 50 µg | $926 | 20 days | 20 days | |
| 100 µg | $1,500 | 20 days | 20 days | |
| 200 µg | $1,750 | 20 days | 20 days | |
| 500 µg | $2,150 | 20 days | 20 days |
| Bioactivity | Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| Description | RUNX1 Protein, Rat, Recombinant (His & Myc) is expressed in Baculovirus insect cells with N-10xHis and C-Myc tag. The predicted molecular weight is 52.4 kDa and the accession number is Q63046. |
| Species | Rat |
| Expression System | Baculovirus Insect Cells |
| Tag | N-10xHis, C-Myc |
| Accession Number | Q63046 |
| Amino Acid | MRIPVDASTSRRFTPPSTALSPGKMSEALPLGAPDGGAALASKLRSGDRSMVEVLADHPGELVRTDSPNFLCSVLPTHWRCNKTLPIAFKVVALGDVPDGTLVTVMAGNDENYSAELRNATAAMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKITVDGPREPRRHRQKLDDQTKPGSLSFSERLSELEQLRRTAMRVSPHHPAPTPNPRASLNHSTAFNPQPQSQMQDARQIQPSPPWSYDQSYQYLGSITSSVHPATPISPGRASGMTSLSAELSSRLSTAPDLTAFGDPRQFPTLPSISDPRMHYPGAFTYSPPVTSGIGIGMSAMSSTSRYHTYLPPPYPGSSQAQAGPFQTGSPSYHLYYGTSAGSYQFSMVGGERSPPRILPPCTNASTGAALLNPSLPSQSDVVETEGSHSNSPTNMPPARLEEAVWRPY |
| Construction | 1-450 aa |
| Protein Purity | > 85% as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Tris-based buffer, 50% glycerol |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Synonyms | Runx1, Runt-related transcription factor 1, Polyomavirus enhancer-binding protein 2 alpha B subunit (PEA2-alpha B;PEBP2-alpha B), Oncogene AML-1, Core-binding factor subunit alpha-2 (CBF-alpha-2), Cbfa2, Aml1, Acute myeloid leukemia 1 protein |
| Research Background | CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter. Inhibits KAT6B-dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells. Positively regulates the expression of RORC in T-helper 17 cells. |
| Molecular Weight | 52.4 kDa (predicted) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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