RNF43 Protein, Human, Recombinant (His)

Name: RNF43 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-04153
Price: 43 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: URCC, RNF43, RNF124, ring finger protein 43) Copy Product Info

Synonyms: URCC, RNF43, RNF124, ring finger protein 43

Catalog No. TMPY-04153 Copy Product Info

Purity: 98.7%

RNF43 mutations are frequently detected in colorectal cancer cells and lead to a loss of function of the ubiquitin E3 ligase. The outer mitochondrial membrane 34 (TOMM34) and ring finger protein 43 (RNF43) as highly expressed oncogenes in malignant colorectal tumors. RNF43 is a tumour suppressor gene that suppresses the Wnt-beta-catenin signalling pathway. RNF43 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.5 kDa and the accession number is Q68DV7-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$43	-	In Stock
10 µg	$66	-	In Stock
20 µg	$105	-	In Stock
50 µg	$196	-	In Stock
100 µg	$333	-	In Stock
200 µg	$573	7-10 days	7-10 days
500 µg	$1,160	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:98.7%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Label Recombinant Human R-Spondin 1/RSPO1 Protein (Cat#TMPY-03626) with biotin. Loaded Biotinylated Human R-Spondin 1/RSPO1 Protein on SA Biosensor, can bind Recombinant Human RNF43 Protein, His Tag (Cat#TMPY-04153) with an affinity constant of 1.37 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested).
Description	RNF43 mutations are frequently detected in colorectal cancer cells and lead to a loss of function of the ubiquitin E3 ligase. The outer mitochondrial membrane 34 (TOMM34) and ring finger protein 43 (RNF43) as highly expressed oncogenes in malignant colorectal tumors. RNF43 is a tumour suppressor gene that suppresses the Wnt-beta-catenin signalling pathway. RNF43 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.5 kDa and the accession number is Q68DV7-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q68DV7-1
Construction	A DNA sequence encoding the human RNF43 (NP_060233.3) (Met1-Tyr197) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Gly 24
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	URCC, RNF43, RNF124, ring finger protein 43
Research Background	RNF43 mutations are frequently detected in colorectal cancer cells and lead to a loss of function of the ubiquitin E3 ligase. The outer mitochondrial membrane 34 (TOMM34) and ring finger protein 43 (RNF43) as highly expressed oncogenes in malignant colorectal tumors. RNF43 is a tumour suppressor gene that suppresses the Wnt-beta-catenin signalling pathway.

Chemical Properties

Molecular Weight

20.5 kDa (predicted); 27 and 23.8 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.