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Synonyms: RIFK, riboflavin kinase

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $77 | - | In Stock | |
| 10 µg | $123 | - | In Stock | |
| 20 µg | $197 | - | In Stock | |
| 50 µg | $398 | - | In Stock |
| Bioactivity | Kinase activity untested |
| Description | Flavokinase is a member of the transferases family, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. Flavokinase is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF, through the activation of the flavokinase gene, enhances the incorporation of FAD in NADPH oxidase enzymes, which is a critical step for the assembly and activation of NADPH oxidase. |
| Species | Human |
| Expression System | E. coli |
| Tag | N-His |
| Accession Number | Q969G6 |
| Construction | A DNA sequence encoding the mature form of human RFK (Q969G6) (Met1-His155) was expressed with a polyhistide tag at the N-terminus. Predicted N terminal: His |
| Protein Purity | > 90 % as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Formulation | Supplied as sterile 20 mM Tris, 10% glycerol, pH 8.0. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Synonyms | RIFK, riboflavin kinase |
| Research Background | Flavokinase is a member of the transferases family, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. Flavokinase is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF, through the activation of the flavokinase gene, enhances the incorporation of FAD in NADPH oxidase enzymes, which is a critical step for the assembly and activation of NADPH oxidase. |
| Molecular Weight | 19.5 kDa (predicted); 20 kDa (reducing conditions) |
| Shipping | Proteins are shipped with blue ice. |
| Storage | It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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