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RFK Protein, Human, Recombinant (His)

(Synonyms: RIFK, riboflavin kinase) Copy Product Info

Synonyms: RIFK, riboflavin kinase

Catalog No. TMPY-04475 Copy Product Info
Flavokinase is a member of the transferases family, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. Flavokinase is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF, through the activation of the flavokinase gene, enhances the incorporation of FAD in NADPH oxidase enzymes, which is a critical step for the assembly and activation of NADPH oxidase.
RFK Protein, Human, Recombinant (His)
Pack SizePriceUSA StockGlobal StockQuantity
5 µg$77-In Stock
10 µg$123-In Stock
20 µg$197-In Stock
50 µg$398-In Stock
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Appearance:Solution
Color:Transparent
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Product Introduction

Bioactivity
Bioactivity
Kinase activity untested
Description
Flavokinase is a member of the transferases family, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. Flavokinase is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF, through the activation of the flavokinase gene, enhances the incorporation of FAD in NADPH oxidase enzymes, which is a critical step for the assembly and activation of NADPH oxidase.
Species
Human
Expression System
E. coli
TagN-His
Accession NumberQ969G6
ConstructionA DNA sequence encoding the mature form of human RFK (Q969G6) (Met1-His155) was expressed with a polyhistide tag at the N-terminus. Predicted N terminal: His
Protein Purity
> 90 % as determined by SDS-PAGE
EndotoxinPlease contact us for more information.
FormulationSupplied as sterile 20 mM Tris, 10% glycerol, pH 8.0.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsRIFK, riboflavin kinase
Research Background
Flavokinase is a member of the transferases family, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. Flavokinase is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF, through the activation of the flavokinase gene, enhances the incorporation of FAD in NADPH oxidase enzymes, which is a critical step for the assembly and activation of NADPH oxidase.
Chemical Properties
Molecular Weight19.5 kDa (predicted); 20 kDa (reducing conditions)
Storage & Solubility Information
ShippingProteins are shipped with blue ice.
StorageIt is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: RFK Protein, Human, Recombinant (His) chemical structure | RFK Protein, Human, Recombinant (His) molecular weight