REG4 Protein, Mouse, Recombinant (His)

Name: REG4 Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-01805
Price: 70 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: RELP, regenerating islet-derived family, member 4, GISP, 2010002L15Rik) Copy Product Info

Synonyms: RELP, regenerating islet-derived family, member 4, GISP, 2010002L15Rik

Catalog No. TMPY-01805 Copy Product Info

REG4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 17.5 kDa and the accession number is Q9D8G5.

Pack Size	Price	USA Stock	Global Stock
5 µg	$70	-	In Stock
10 µg	$109	-	In Stock
20 µg	$182	-	In Stock
50 µg	$357	7-10 days	7-10 days
100 µg	$600	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:> 95 % as determined by SDS-PAGE

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by the ability of the immobilized protein to support the adhesion and proliferation of HCT-116 human colorectal carcinoma cells (ATCC: CCL-247) under low serum conditions. When 5 x 10E4 cells/well are added to mouse REG4 coated plates (0.16 μg/ml and 100 μl/well), >70% cell will adhere after 60 minutes at 37℃.
Description	REG4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 17.5 kDa and the accession number is Q9D8G5.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q9D8G5
Construction	A DNA sequence encoding mouse REG4 (NP_080604.2) (Met 1-Thr 157) was expressed with the polyhistide tag at the C-terminus. Predicted N terminal: Asp 23
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	RELP, regenerating islet-derived family, member 4, GISP, 2010002L15Rik
Research Background	Regenerating islet-derived protein 4, also known as REG-like protein, REG4, GISP and RELP, a member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily, has been found to be involved in malignancy in several different organs including the stomach, colorectum, pancreas and prostate. It is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. Expression of the Reg4 in different cell types has been associated with regeneration, cell growth and cell survival, cell adhesion and resistance to apoptosis. REG4 protein overexpression is associated with an unfavorable response to preoperative chemoradiotherapy and may be used as a predictive biomarker clinically. REG4 may play an important role in the development and progression of colorectal cancer, as well as in intestinal morphogenesis and epithelium restitution.

Chemical Properties

Molecular Weight

17.5 kDa (predicted); 23-30 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.