PTPN12 Protein, Human, Recombinant (aa 1-355, His & GST)

Catalog No. TMPY-03486

PTPN12 Protein, Human, Recombinant (aa 1-355, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 69.4 kDa and the accession number is AAA36529.1.

PTPN12 Protein, Human, Recombinant (aa 1-355, His & GST)

Catalog No. TMPY-03486

Pack Size	Price	Availability
5 μg	$79	7-10 days
10 μg	$129	7-10 days
20 μg	$208	7-10 days
50 μg	$419	7-10 days
100 μg	$812	7-10 days

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Product Information

Biological Activity	Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate. The specific activity is > 15 μmoles/min/mg.
Description	PTPN12 Protein, Human, Recombinant (aa 1-355, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 69.4 kDa and the accession number is AAA36529.1.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	His, GST
Accession Number	AAA36529.1
Synonyms	PTP-PEST,PTPG1,protein tyrosine phosphatase, non-receptor type 12
Construction	A DNA sequence encoding the human PTPN12 (AAA36529.1) (Met1-Gln355) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus. Predicted N terminal: Met
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	69.4 kDa (predicted); 64 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% gly, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	PTPN12 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN12 contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may be related to protein intracellular half-life. PTPN12 was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. PTPN12 was shown to interact with, and dephosphorylate, various cytoskeleton and cell adhesion molecules, such as p13 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobility.