PRMT5 Protein, Human, Recombinant (Flag & His)

Name: PRMT5 Protein, Human, Recombinant (Flag & His)
Brand: TargetMol
SKU: TMPY-07051
Rating: 4.5 (1 reviews)

(Synonyms: SKB1Hs, SKB1, JBP1, IBP72, HSL7, HRMT1L5) Copy Product Info

Synonyms: SKB1Hs, SKB1, JBP1, IBP72, HSL7, HRMT1L5

Catalog No. TMPY-07051 Copy Product Info

PRMT5 Protein, Human, Recombinant (Flag & His) is expressed in Baculovirus-Insect Cells. The accession number is O14744-1.

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Pack Size	Price	USA Stock	Global Stock
10 µg	$126	7-10 days	7-10 days
20 µg	$198	7-10 days	7-10 days
50 µg	$397	7-10 days	7-10 days
100 µg	$796	7-10 days	7-10 days
200 µg	$1,200	7-10 days	7-10 days
500 µg	$2,590	7-10 days	7-10 days
1 mg	$5,280	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	PRMT5 Protein, Human, Recombinant (Flag & His) is expressed in Baculovirus-Insect Cells. The accession number is O14744-1.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	N-His-Flag
Accession Number	O14744-1
Construction	A DNA sequence encoding the Human PRMT5 (NP_006100.2) (Ala2-Leu637) was expressed with a N-terminal polyhistide-tagged FLAG tag at the N-terminus (his-FLAG).
Protein Purity	≥ 90% as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 300mM NaCl, 25% Glycerol,1mM TCEP, pH 8.0. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Synonyms	SKB1Hs, SKB1, JBP1, IBP72, HSL7, HRMT1L5
Research Background	Methylation of arginine residues is a widespread post-translational modification of proteins catalyzed by a small family of PRMTs. The modification appears to regulate protein functions and interactions that affect gene regulation, signalling and subcellular localization of proteins and nucleic acids. Protein arginine methyltransferase 5 (PRMT5) is a member of the protein arginine N-methyltransferases (PRMT)family, and exists as at least homodimers and homotetramers, or homooligomers mediated by disulfide bonds and non-covalent association ubiquitously. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), and thus plays a role in the assembly and biogenesis of snRNP core particles. PRMT5 methylates histone H2A and H4 'Arg-3' during germ cell development, as well as histone H3 'Arg-8', which may repress transcription. PRMT5 also methylates SUPT5H and regulates its transcriptional elongation properties. Additionally, it is also suggested that PRMT5 negatively regulates cyclin E1 promoter activity and cellular proliferation.

Chemical Properties

Molecular Weight

75.06 kDa (predicted); 64.8 kDa (reducing contition, due to glycosylation)

Storage & Solubility Information

Shipping	Proteins are shipped with blue ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.