PRAME Protein, Human, Recombinant (His)

Catalog No. TMPH-01651

PRAME Protein, Human, Recombinant (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 59.9 kDa and the accession number is P78395.

PRAME Protein, Human, Recombinant (His)

Catalog No. TMPH-01651

PRAME Protein, Human, Recombinant (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 59.9 kDa and the accession number is P78395.

Pack Size	Price	Availability
5 μg	$86	20 days
10 μg	$138	20 days
20 μg	$231	20 days
50 μg	$348	20 days
100 μg	$480	20 days
200 μg	$743	20 days
500 μg	$1,330	20 days

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Product Information

Biological Activity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	PRAME Protein, Human, Recombinant (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 59.9 kDa and the accession number is P78395.
Species	Human
Expression System	P. pastoris (Yeast)
Tag	N-6xHis
Accession Number	P78395
Synonyms	Preferentially expressed antigen of melanoma,PRAME,Opa-interacting protein 4 (OIP-4),OIP4,Melanoma antigen preferentially expressed in tumors,MAPE
Amino Acid	MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAALELLPRELFPPLFMAAFDGRHSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFKAVLDGLDVLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNRASLYSFPEPEAAQPMTKKRKVDGLSTEAEQPFIPVEVLVDLFLKEGACDELFSYLIEKVKRKKNVLRLCCKKLKIFAMPMQDIKMILKMVQLDSIEDLEVTCTWKLPTLAKFSPYLGQMINLRRLLLSHIHASSYISPEKEEQYIAQFTSQFLSLQCLQALYVDSLFFLRGRLDQLLRHVMNPLETLSITNCRLSEGDVMHLSQSPSVSQLSVLSLSGVMLTDVSPEPLQALLERASATLQDLVFDECGITDDQLLALLPSLSHCSQLTTLSFYGNSISISALQSLLQHLIGLSNLTHVLYPVPLESYEDIHGTLHLERLAYLHARLRELLCELGRPSMVWLSANPCPHCGDRTFYDPEPILCPCFMPN
Construction	1-509 aa
Protein Purity	> 90% as determined by SDS-PAGE.
Molecular Weight	59.9 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Tris-based buffer, 50% glycerol
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation. The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding. In the nucleus, the CRL2(PRAME) complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY) and probably plays a role in chromstin regulation. Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG: prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.