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Porin MspA Protein, Mycobacterium smegmatis, Recombinant (His)

(Synonyms: Porin MspA, mspA) Copy Product Info

Synonyms: Porin MspA, mspA

Catalog No. TMPH-02998 Copy Product Info
Porin MspA Protein, Mycobacterium smegmatis, Recombinant (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 25.4 kDa and the accession number is A0QR29.
Porin MspA Protein, Mycobacterium smegmatis, Recombinant (His)
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Pack SizePriceUSA StockGlobal StockQuantity
5 µg$129-In Stock
10 µg$216-In Stock
20 µg$36020 days20 days
50 µg$54320 days20 days
100 µg$74520 days20 days
200 µg$1,07020 days20 days
500 µg$1,73020 days20 days
1 mg$2,53020 days20 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:93%
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Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Porin MspA Protein, Mycobacterium smegmatis, Recombinant (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 25.4 kDa and the accession number is A0QR29.
Species
Mycobacterium smegmatis
Expression System
E. coli
TagN-10xHis
Accession NumberA0QR29
Amino AcidGLDNELSLVDGQDRTLTVQQWDTFLNGVFPLDRNRLTREWFHSGRAKYIVAGPGADEFEGTLELGYQIGFPWSLGVGINFSYTTPNILIDDGDITAPPFGLNSVITPNLFPGVSISADLGNGPGIQEVATFSVDVSGAEGGVAVSNAHGTVTGAAGGVLLRPFARLIASTGDSVTTYGEPWNMN
Construction28-211 aa
Protein Purity
> 85% as determined by SDS-PAGE.
Porin MspA Protein, Mycobacterium smegmatis, Recombinant (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, pH 8.0, 50% glycerol
SynonymsPorin MspA, mspA
Research Background
The major porin in this organism, forms a water-filled channel which favors the permeation of cations, amino acids, iron Fe(3+) and less efficiently phosphate. Does not transport Fe-ExoMS, the predominant siderophore. Plays a role in transport of beta-lactamase and hydrophilic fluoroquinolone antibiotics such as norfloxacin as well as chloramphenicol. There are about 2400 porins in wild-type, 800 in an mspA deletion and 150 in a double mspA-mspC deletion. Different conductance values with maxima at 2.3 and 4.6 nanosiemens might be caused by a simultaneous reconstitution of MspA channels into the membrane or by the existence of different MspA conformations.
Chemical Properties
Molecular Weight25.4 kDa (predicted)
Storage & Solubility Information
ShippingProteins are shipped with blue ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: Porin MspA Protein, Mycobacterium smegmatis, Recombinant (His) chemical structure | Porin MspA Protein, Mycobacterium smegmatis, Recombinant (His) molecular weight