Protein O-Linked-Mannose β-1 2-N-Acetylglucosaminyltransferase 1 (POMGNT1) belongs to the Glycosyltransferase 13 family. Amino acid residues between 299-311 are important for both protein expression and enzymatic activity. The minimal catalytic domain is located between positions 299-651. It is suggested that the stem domain of the soluble form is unnecessary for activity, but that some amino acids play a crucial role in the membrane-bound form. Defects in POMGNT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies type A3 (MDDGA3).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
10 μg | 5 days | $ 184.00 | |
50 μg | 5 days | $ 545.00 | |
500 μg | 5 days | $ 1,900.00 | |
1 mg | 5 days | $ 2,730.00 |
Description | Protein O-Linked-Mannose β-1 2-N-Acetylglucosaminyltransferase 1 (POMGNT1) belongs to the Glycosyltransferase 13 family. Amino acid residues between 299-311 are important for both protein expression and enzymatic activity. The minimal catalytic domain is located between positions 299-651. It is suggested that the stem domain of the soluble form is unnecessary for activity, but that some amino acids play a crucial role in the membrane-bound form. Defects in POMGNT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies type A3 (MDDGA3). |
Species | Human |
Expression System | Human Cells |
Tag | C-6His |
Accession Number | Q8WZA1 |
Synonyms | 2-N-Acetylglucosaminyltransferase 1, UDP-GlcNAc:α-D-Mannoside β-1, 2-N-Acetylglucosaminyltransferase I.2, Protein O-Linked-Mannose β-1, UDP-GlcNAc:Alpha-D-Mannoside β-1, POMGNT1, MGAT1.2, Protein O-Linked-Mannose Beta-1, UDP-GlcNAc:Alpha-D-Mannoside Beta-1 |
Amino Acid | Leu59-Thr660 |
Construction | Recombinant Human Protein O-Linked-Mannose Beta-1 2-N-Acetylglucosaminyltransferase 1 is produced by our Mammalian expression system and the target gene encoding Leu59-Thr660 is expressed with a 6His tag at the C-terminus. |
Molecular Weight | 74 KDa, reducing conditions |
Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5. |
Stability & Storage |
Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
Shipping |
The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
Research Background | Protein O-Linked-Mannose β-1 2-N-Acetylglucosaminyltransferase 1 (POMGNT1) belongs to the Glycosyltransferase 13 family. Amino acid residues between 299-311 are important for both protein expression and enzymatic activity. The minimal catalytic domain is located between positions 299-651. It is suggested that the stem domain of the soluble form is unnecessary for activity, but that some amino acids play a crucial role in the membrane-bound form. Defects in POMGNT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies type A3 (MDDGA3). |
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POMGNT1 Protein, Human, Recombinant (His) POMGNT-1 2-N-Acetylglucosaminyltransferase 1 UDP-GlcNAc:α-D-Mannoside β-1 2-N-Acetylglucosaminyltransferase I.2 Protein O-Linked-Mannose β-1 Protein O-Linked-Mannose b-1 UDP-GlcNAc:a-D-Mannoside b-1 UDP-GlcNAc:Alpha-D-Mannoside β-1 POMGNT1 POMGNT 1 MGAT1.2 Protein O-Linked-Mannose Beta-1 UDP-GlcNAc:Alpha-D-Mannoside Beta-1 recombinant recombinant-proteins proteins protein