PKLR Protein, Human, Recombinant (His)

Name: PKLR Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-04765
Rating: 4.5 (1 reviews)

(Synonyms: RPK, pyruvate kinase, liver and RBC, PKRL, PKR, PKL, PK1) Copy Product Info

Synonyms: RPK, pyruvate kinase, liver and RBC, PKRL, PKR, PKL, PK1

Catalog No. TMPY-04765 Copy Product Info

Pyruvate kinase (PKLR) is a critical erythrocyte enzyme that is required for glycolysis and production of ATP. Pyruvate kinase deficiency (PKD) is the most frequent red blood cell enzyme abnormality of the glycolytic pathway and the most common cause of hereditary nonspherocytic hemolytic anemia. Over 250 PKLR-gene mutations have been described, including missense/nonsense, splicing and regulatory mutations, small insertions, small and gross deletions, causing PKD and hemolytic anemia of variable severity. PKLR expression was increased in liver metastases as well as in primary colorectal tumors of patients with metastatic disease. PKLR protein variants may affect the frequency, and the intensity of malaria episodes induced by different Plasmodium parasites in humans living in areas of endemic malaria.

Pack Size	Price	USA Stock	Global Stock
10 µg	$118	7-10 days	7-10 days
20 µg	$197	7-10 days	7-10 days
50 µg	$458	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Kinase activity untested
Description	Pyruvate kinase (PKLR) is a critical erythrocyte enzyme that is required for glycolysis and production of ATP. Pyruvate kinase deficiency (PKD) is the most frequent red blood cell enzyme abnormality of the glycolytic pathway and the most common cause of hereditary nonspherocytic hemolytic anemia. Over 250 PKLR-gene mutations have been described, including missense/nonsense, splicing and regulatory mutations, small insertions, small and gross deletions, causing PKD and hemolytic anemia of variable severity. PKLR expression was increased in liver metastases as well as in primary colorectal tumors of patients with metastatic disease. PKLR protein variants may affect the frequency, and the intensity of malaria episodes induced by different Plasmodium parasites in humans living in areas of endemic malaria.
Species	Human
Expression System	E. coli
Tag	N-His
Accession Number	P30613-1
Construction	A DNA sequence encoding the human PKLR (NP_000289.1) (Leu47-Ser574) was expressed with a polyhistidine tag at the N-terminus. Predicted N terminal: His
Protein Purity	> 85 % as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile PBS, pH 7.4.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	RPK, pyruvate kinase, liver and RBC, PKRL, PKR, PKL, PK1
Research Background	Pyruvate kinase (PKLR) is a critical erythrocyte enzyme that is required for glycolysis and production of ATP. Pyruvate kinase deficiency (PKD) is the most frequent red blood cell enzyme abnormality of the glycolytic pathway and the most common cause of hereditary nonspherocytic hemolytic anemia. Over 250 PKLR-gene mutations have been described, including missense/nonsense, splicing and regulatory mutations, small insertions, small and gross deletions, causing PKD and hemolytic anemia of variable severity. PKLR expression was increased in liver metastases as well as in primary colorectal tumors of patients with metastatic disease. PKLR protein variants may affect the frequency, and the intensity of malaria episodes induced by different Plasmodium parasites in humans living in areas of endemic malaria.

Chemical Properties

Molecular Weight

59.1 kDa (predicted); 53-60 kDa (reducing conditions)

Storage & Solubility Information

Shipping	Proteins are shipped with blue ice.
Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.