6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 is an enzyme that in humans is encoded by the PFKFB1 gene. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. It belongs to the phosphoglycerate mutase family. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
10 μg | 5 days | $ 184.00 | |
50 μg | 5 days | $ 545.00 | |
500 μg | 5 days | $ 1,900.00 | |
1 mg | 5 days | $ 2,970.00 |
Description | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 is an enzyme that in humans is encoded by the PFKFB1 gene. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. It belongs to the phosphoglycerate mutase family. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. |
Species | Human |
Expression System | Human Cells |
Tag | C-6His |
Accession Number | P16118 |
Synonyms | PFKFB1, F6PK, 6-P2ase liver isozyme, 6-bisphosphatase 1, 6PF-2-K/Fru-2, 6-bisphosphatase, PFRX, Fructose-2, 6-phosphofructo-2-kinase/fructose-2 |
Amino Acid | Ser2-Tyr471 |
Construction | Recombinant Human 6-Phosphofructo-2-kinase/Fructose-2,6-bisphosphatase 1 is produced by our Mammalian expression system and the target gene encoding Ser2-Tyr471 is expressed with a 6His tag at the C-terminus. |
Protein Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
Molecular Weight | 60 KDa, reducing conditions |
Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, 1mM EDTA, pH 7.8. |
Stability & Storage |
Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
Shipping |
The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
Research Background | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 is an enzyme that in humans is encoded by the PFKFB1 gene. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. It belongs to the phosphoglycerate mutase family. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. |
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PFKFB1 Protein, Human, Recombinant (His) PFKFB 1 PFKFB1 F6PK 6-P2ase liver isozyme 6-bisphosphatase 1 6PF-2-K/Fru-2 6-bisphosphatase PFRX Fructose-2 6-phosphofructo-2-kinase/fructose-2 PFKFB-1 recombinant recombinant-proteins proteins protein