ED 50 < 2.0 ng/ml, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 5.0 × 10 5 units/mg.

PDGF-BB Protein, Rat, Recombinant is expressed in E. coli. The accession number is Q05028.

Rat

E. coli

SSV,SIS,platelet derived growth factor subunit B,PDGFBB,PDGF-2,PDGF2,IBGC5,c-sis

Ser74-Thr182

> 95% as determined by SDS-PAGE

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in 20 mM acetic acid up to 100 μg/ml.

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.