ED 50 < 20.00 ng/ml as determined by a dose-response proliferation assay using murine Balb/c 3T3 cells. Based on the ED 50 , the calculated specific activity is approximately > 0.5 × 10 5 IU/mg. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay.

PDGF-BB Protein, Bovine, Recombinant is expressed in P. pastoris (Yeast). The accession number is B1H0W5.

Bovine

P. pastoris (Yeast)

PDGF-BB,PDGFBB

Ser82-Thr190

≥ 95% as determined by SDS-PAGE

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product is stable for up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is storage at -20 °C.

Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in an animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, and PDGF-AB. It is involved in many biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β).