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PDGF-BB Protein, Bovine, Recombinant
(Synonyms: PDGF-BB, PDGFBB) Copy Product InfoSynonyms: PDGF-BB, PDGFBB
Catalog No. TMPS-00115 Copy Product Info
PDGF-BB Protein, Bovine, Recombinant is expressed in P. pastoris (Yeast). The accession number is B1H0W5.
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| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $148 | 7-10 days | 7-10 days | |
| 10 µg | $247 | 7-10 days | 7-10 days | |
| 20 µg | $398 | 7-10 days | 7-10 days | |
| 50 µg | $755 | 7-10 days | 7-10 days | |
| 100 µg | $1,230 | 7-10 days | 7-10 days | |
| 200 µg | $1,970 | 7-10 days | 7-10 days | |
| 500 µg | $3,680 | 7-10 days | 7-10 days | |
| 1 mg | $5,970 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | ED 50 < 20.00 ng/ml as determined by a dose-response proliferation assay using murine Balb/c 3T3 cells. Based on the ED 50 , the calculated specific activity is approximately > 0.5 × 10 5 IU/mg. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay. |
| Description | PDGF-BB Protein, Bovine, Recombinant is expressed in P. pastoris (Yeast). The accession number is B1H0W5. |
| Species | Bovine |
| Expression System | P. pastoris (Yeast) |
| Tag | Tag Free |
| Accession Number | B1H0W5 |
| Construction | Ser82-Thr190 |
| Protein Purity | ≥ 95% as determined by SDS-PAGE |
| Endotoxin | < 0.1 EU/μg of protein as determined by the LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in 20 mM NaAc, pH 5.0. |
| Reconstitution | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | PDGF-BB, PDGFBB |
| Research Background | Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in an animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, and PDGF-AB. It is involved in many biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β). |
| Molecular Weight | 12 kDa (Predicted); 10-18 kDa (Reducing conditions) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product is stable for up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is storage at -20 °C. |
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Related Tags: PDGF-BB Protein, Bovine, Recombinant chemical structure | PDGF-BB Protein, Bovine, Recombinant molecular weight
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