PDGF-AA Protein, Rat, Recombinant (His)

Name: PDGF-AA Protein, Rat, Recombinant (His)
Brand: TargetMol
SKU: TMPK-02004
Rating: 4.5 (1 reviews)

(Synonyms: Rpa1, PDGF-AA, PDGFAA, PDGF-A, Pdgfa, PDGF-1, PDGF1, PDGF AA, PDGF A) Copy Product Info

Synonyms: Rpa1, PDGF-AA, PDGFAA, PDGF-A, Pdgfa, PDGF-1, PDGF1, PDGF AA, PDGF A

Catalog No. TMPK-02004 Copy Product Info

PDGF-AA Protein, Rat, Recombinant (His) is expressed in P. pastoris (Yeast). The accession number is P28576-1.

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Pack Size	Price	USA Stock	Global Stock
5 µg	$74	7-10 days	7-10 days
10 µg	$113	7-10 days	7-10 days
20 µg	$175	7-10 days	7-10 days
50 µg	$316	7-10 days	7-10 days
100 µg	$490	7-10 days	7-10 days
200 µg	$793	7-10 days	7-10 days
500 µg	$1,470	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 5-15 ng/ml.
Description	PDGF-AA Protein, Rat, Recombinant (His) is expressed in P. pastoris (Yeast). The accession number is P28576-1.
Species	Rat
Expression System	P. pastoris (Yeast)
Tag	N-His
Accession Number	P28576-1
Construction	Arg86-Lys204
Protein Purity	> 90% as determined by Bis-Tris PAGE
Endotoxin	< 1 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	Rpa1, PDGF-AA, PDGFAA, PDGF-A, Pdgfa, PDGF-1, PDGF1, PDGF AA, PDGF A
Research Background	PDGF (Platelet-derived growth factor) is one among numerous growth factors that regulate cell growth and division. The PDGF family forms five dimeric ligands, PDGF-AA, PDGF-BB, PDGF-CC, PDGF-DD, and PDGF-AB, which activate cognate receptor tyrosine kinases PDGFR-α and PDGFR-β. Upon binding to its tyrosine kinase receptor PDGF-αα, PDGF-AA stimulates the proliferation of osteoblastic cells and may exert autocrine and paracrine effects in regulating bone-forming processes. PDGF-AA is the most well characterized mitogen that is not restricted to any kind of tumor cell type but was seen in osteoblastic cells as well as in pleomorphic tumor elements.

Chemical Properties

Molecular Weight

14.75 kDa (Predicted); 17-22 kDa and 25-45 kDa (Reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.