PARP2 Protein, Human, Recombinant (His)

Name: PARP2 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPH-01880
Rating: 4.5 (1 reviews)

(Synonyms: Protein poly-ADP-ribosyltransferase PARP2, Poly[ADP-ribose] synthase 2 (pADPRT-2), Poly [ADP-ribose] polymerase 2, PARP-2, PARP2, NAD(+) ADP-ribosyltransferase 2 (ADPRT-2), hPARP-2, DNA ADP-ribosyltransferase PARP2, ADPRTL2, ADPRT2, ADP-ribosyltransferase diphtheria toxin-like 2 (ARTD2)) Copy Product Info

Synonyms: Protein poly-ADP-ribosyltransferase PARP2, Poly[ADP-ribose] synthase 2 (pADPRT-2), Poly [ADP-ribose] polymerase 2, PARP-2, PARP2, NAD(+) ADP-ribosyltransferase 2 (ADPRT-2), hPARP-2, DNA ADP-ribosyltransferase PARP2, ADPRTL2, ADPRT2, ADP-ribosyltransferase diphtheria toxin-like 2 (ARTD2)

Catalog No. TMPH-01880 Copy Product Info

PARP2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 72.2 kDa and the accession number is Q9UGN5.

Pack Size	Price	USA Stock	Global Stock
5 µg	$105	20 days	20 days
10 µg	$169	20 days	20 days
20 µg	$283	20 days	20 days
50 µg	$428	20 days	20 days
100 µg	$590	20 days	20 days
200 µg	$913	20 days	20 days
500 µg	$1,620	20 days	20 days
1 mg	$2,530	20 days	20 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	PARP2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 72.2 kDa and the accession number is Q9UGN5.
Species	Human
Expression System	E. coli
Tag	C-10xHis
Accession Number	Q9UGN5
Amino Acid	QLDLRVQELIKLICNVQAMEEMMMEMKYNTKKAPLGKLTVAQIKAGYQSLKKIEDCIRAGQHGRALMEACNEFYTRIPHDFGLRTPPLIRTQKELSEKIQLLEALGDIEIAIKLVKTELQSPEHPLDQHYRNLHCALRPLDHESYEFKVISQYLQSTHAPTHSDYTMTLLDLFEVEKDGEKEAFREDLHNRMLLWHGSRMSNWVGILSHGLRIAPPEAPITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEVALGQCNELLEANPKAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDGYTLNYNEYIVYNPNQVRMRYLLKVQFNFLQLW
Construction	233-583 aa
Protein Purity	> 85% as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	Protein poly-ADP-ribosyltransferase PARP2, Poly[ADP-ribose] synthase 2 (pADPRT-2), Poly [ADP-ribose] polymerase 2, PARP-2, PARP2, NAD(+) ADP-ribosyltransferase 2 (ADPRT-2), hPARP-2, DNA ADP-ribosyltransferase PARP2, ADPRTL2, ADPRT2, ADP-ribosyltransferase diphtheria toxin-like 2 (ARTD2)
Research Background	Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site. PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks. In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex.

Chemical Properties

Molecular Weight

41.3 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.