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Synonyms: Poly-PARP, PARS, PARP-1, PARP, pADPRT-1, ARTD1, ADPRT1, ADPRT 1, ADPRT


| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $208 | 7-10 days | 7-10 days | |
| 10 μg | $343 | 7-10 days | 7-10 days | |
| 20 μg | $588 | 7-10 days | 7-10 days | |
| 50 μg | $939 | 7-10 days | 7-10 days | |
| 100 μg | $1,600 | 7-10 days | 7-10 days |
| Description | PARP1 Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in Baculovirus-Insect Cells. The accession number is P09874. |
| Species | Human |
| Expression System | Baculovirus Insect Cells |
| Tag | C-His-Avi |
| Accession Number | P09874 |
| Construction | A DNA sequence encoding the Human PARP1 (NP_001609.2)(Met1-Trp1014) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
| Protein Purity | ≥ 90% as determined by SDS-PAGE. ≥ 90% as determined by SEC-HPLC. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile 20 mM Tris, 300mM NaCl, 10% glycerol, 0.5mM TCEP, 2mM EDTA, pH 7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Please refer to the lot-specific COA. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | Poly-PARP, PARS, PARP-1, PARP, pADPRT-1, ARTD1, ADPRT1, ADPRT 1, ADPRT |
| Research Background |
| Molecular Weight | 116.35 kDa (predicted); 104.5 kDa (reducing contition) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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