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Orange fluorescent protein Protein, Discosoma sp, Recombinant (His)
(Synonyms: OFP Protein) Copy Product InfoSynonyms: OFP Protein
Catalog No. TMPY-04336 Copy Product Info
OFPSparkTM is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSparkTM is more than twice brighter than mOrange2. Fast OFPSparkTM maturation makes it detectable in mammalian cells as early as within 8 hrs after transfection. OFPSparkTM can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with OFPSparkTM expression vectors produce bright fluorescence in 8 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. For its monomer structure, OFPSparkTM performs well in some fusions and protein labeling applications.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $108 | 7-10 days | 7-10 days | |
| 10 µg | $178 | 7-10 days | 7-10 days | |
| 20 µg | $296 | 7-10 days | 7-10 days | |
| 50 µg | $696 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Information
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | OFPSparkTM expression vector transfected 293 H cells transiently. After 48 h, the strong orange fluorescent signals can be detected under the excitation channel of 503.5~547.5 nm, and the strong red fluorescent signals can be detected under the excitation channel of 532.5~587.5 nm. |
| Description | OFPSparkTM is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSparkTM is more than twice brighter than mOrange2. Fast OFPSparkTM maturation makes it detectable in mammalian cells as early as within 8 hrs after transfection. OFPSparkTM can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with OFPSparkTM expression vectors produce bright fluorescence in 8 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. For its monomer structure, OFPSparkTM performs well in some fusions and protein labeling applications. |
| Species | Discosoma sp |
| Expression System | E. coli |
| Tag | C-His |
| Construction | OFPSparkTM is a red (orange) fluorescent protein derived from DsRed (similarity is 98%). OFPSparkTM was expressed with a polyhistide tag at the C-terminus (Patent 201510003374.3). Predicted N terminal: Met |
| Protein Purity | > 95 % as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing Tris, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Synonyms | OFP Protein |
| Research Background | OFPSparkTM is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSparkTM is more than twice brighter than mOrange2. Fast OFPSparkTM maturation makes it detectable in mammalian cells as early as within 8 hrs after transfection. OFPSparkTM can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with OFPSparkTM expression vectors produce bright fluorescence in 8 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. For its monomer structure, OFPSparkTM performs well in some fusions and protein labeling applications. |
| Molecular Weight | 26.4 kDa (predicted) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: Orange fluorescent protein Protein, Discosoma sp, Recombinant (His) chemical structure | Orange fluorescent protein Protein, Discosoma sp, Recombinant (His) molecular weight
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