Beta-NGF Protein, Mouse, Recombinant

Name: Beta-NGF Protein, Mouse, Recombinant
Brand: TargetMol
SKU: TMPY-01402
Price: 64 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: Ngfb, nerve growth factor (β polypeptide), nerve growth factor (beta polypeptide)) Copy Product Info

Synonyms: Ngfb, nerve growth factor (β polypeptide), nerve growth factor (beta polypeptide)

Catalog No. TMPY-01402 Copy Product Info

Purity: 99.1%

NGF Protein, Mouse, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 13.5 kDa and the accession number is P01139.

Pack Size	Price	USA Stock	Global Stock
5 µg	$64	-	In Stock
10 µg	$98	7-10 days	7-10 days
20 µg	$165	In Stock	In Stock
50 µg	$295	7-10 days	7-10 days
100 µg	$462	-	In Stock
200 µg	$791	7-10 days	7-10 days
500 µg	$1,680	7-10 days	7-10 days
1 mg	$3,000	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.1%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-6 ng/mL.
Description	NGF Protein, Mouse, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 13.5 kDa and the accession number is P01139.
Species	Mouse
Expression System	CHO Cells
Tag	Tag Free
Accession Number	P01139
Construction	A DNA sequence encoding the mature form of mouse NGF (NP_001106168.1) (Ser 122-Gly 241) was expressed, with an initial Met at the C-terminus. Predicted N terminal: Met
Protein Purity	> 96 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 150 mM NaCl, 50 mM NaAc, pH 5. 5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	Ngfb, nerve growth factor (β polypeptide), nerve growth factor (beta polypeptide)
Research Background	Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.

Chemical Properties

Molecular Weight

13.5 kDa (predicted); 13.5 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.