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MSLN/Mesothelin Protein, Human, Recombinant (His), AF647-Labeled

TargetMol | SPR
Catalog No. TMPY-07109 Copy Product Info
MSLN/Mesothelin Protein, Human, Recombinant (His), AF647-Labeled is expressed in HEK293 Cells. The accession number is Q13421.

MSLN/Mesothelin Protein, Human, Recombinant (His), AF647-Labeled

Catalog No. TMPY-07109
Copy Product Info
TargetMol | SPR

MSLN/Mesothelin Protein, Human, Recombinant (His), AF647-Labeled is expressed in HEK293 Cells. The accession number is Q13421.

MSLN/Mesothelin Protein, Human, Recombinant (His), AF647-Labeled
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$1637-10 days7-10 days
10 μg$2737-10 days7-10 days
25 μg$5397-10 days7-10 days
50 μg$9397-10 days7-10 days
100 μg$1,9607-10 days7-10 days
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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)
For research use only—not for human use. No sales to individuals. Use as intended only.
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Biological Activity
1. Flow cytometric analysis of anti-Mesothelin CAR expression. 293 cells were transiently transduced with anti-Mesothelin CAR. Flow cytometric analysis was performed with MSLN/Mesothelin Protein, Human, Recombinant (His), AF647-Labeled (Cat#TMPY-07109). Non-transduced 293 cells were used as a control (left).
2. Binding activity of AF 647-conjugated Mesothelin protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and MSLN/Mesothelin Protein, Human, Recombinant (His), AF647-Labeled (Cat#TMPY-07109) and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control (left).
Description
MSLN/Mesothelin Protein, Human, Recombinant (His), AF647-Labeled is expressed in HEK293 Cells. The accession number is Q13421.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberQ13421
Synonyms
SMRP,MPF,CAK1
Construction
A DNA sequence encoding the human MSLN (AAH09272.1) (Glu296-Gly580) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 647 (Excitation Max.= 655 nm, Emission Max.= 680 nm).
Molecular Weight38 kDa (predicted)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationThis product is Lyophilized from sterile PBS, pH 7.4, with 5 %-8 % trehalose. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Please refer to the lot-specific COA.
Stability & Storage
Twelve months from date of receipt at -20℃ to -70℃ in lyophilized form and 3 months at -70℃ under sterile conditions after reconstitution. Protect from prolonged exposure to light and avoid repeated freeze-thaw cycles.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background
The megakaryocyte potentiating factor belongs to the mesothelin family. This family is comprised of several mammalian pre-pro-megakaryocyte potentiating factor precursor (MPF) or mesothelin proteins. Mesothelin is a glycosylphosphatidylinositol-linked glycoprotein highly expressed in mesothelial cells, mesotheliomas, and ovarian cancer, but the biological function of the protein is not known. Megakaryocyte potentiating factor is highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). It interacts with MUC16 and potentiates megakaryocyte colony formation in vitro. Megakaryocyte potentiating factor is secreted by several mesothelioma cell lines and is frequently elevated in the blood of patients with mesothelioma. Measurement of this protein may be useful in following the response of mesothelioma to treatment.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

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