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MGMT Protein, Human, Recombinant (His)

Catalog No. TMPJ-00872
Synonyms: MGMT, 6-O-methylguanine-DNAmethyltransferase, O-6-methylguanine-DNA-alkyltransferase, Methylated-DNA--protein-cysteine methyltransferase

MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.

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MGMT Protein, Human, Recombinant (His)
Pack Size Availability Price/USD Quantity
10 μg 5 days $ 116.00
50 μg 5 days $ 352.00
500 μg 5 days $ 1,900.00
1 mg 5 days $ 2,730.00
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Biological Description
Technical Params
Product Properties
Description MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.
Species Human
Expression System E. coli
Tag N-6His
Accession Number P16455
Synonyms MGMT, 6-O-methylguanine-DNAmethyltransferase, O-6-methylguanine-DNA-alkyltransferase, Methylated-DNA--protein-cysteine methyltransferase
Amino Acid Met1-Asn207
Construction Recombinant Human Methylated-DNA-protein-cysteine Methyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Asn207 is expressed with a 6His tag at the N-terminus.
Protein Purity Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight 25 KDa, reducing conditions
Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl,1mM DTT,1mM EDTA,500mM NaCl,0.1%Trition X-100,pH 8.0.
Stability & Storage

Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Shipping

The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Research Background MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.

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Keywords

MGMT Protein, Human, Recombinant (His) MGMT 6-O-methylguanine-DNAmethyltransferase O-6-methylguanine-DNA-alkyltransferase Methylated-DNA--protein-cysteine methyltransferase recombinant recombinant-proteins proteins protein

 

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