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Lysenin Protein, Eisenia fetida, Recombinant (His)

Name: Lysenin Protein, Eisenia fetida, Recombinant (His)
Brand: TargetMol
SKU: TMPH-00511
Rating: 4.5 (1 reviews)

(Synonyms: Lysenin, Invertebrate cytolysin pore, efL1, Beta-barrel pore-forming toxin (Beta-PFT)) Copy Product Info

Synonyms: Lysenin, Invertebrate cytolysin pore, efL1, Beta-barrel pore-forming toxin (Beta-PFT)

Catalog No. TMPH-00511 Copy Product Info

Lysenin Protein, Eisenia fetida, Recombinant (His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 40.4 kDa and the accession number is O18423.

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Pack Size	Price	USA Stock	Global Stock
5 μg	$129	20 days	20 days
10 μg	$216	20 days	20 days
20 μg	$360	20 days	20 days
50 μg	$543	20 days	20 days
100 μg	$745	20 days	20 days
200 μg	$1,070	20 days	20 days
500 μg	$1,730	20 days	20 days
1 mg	$2,530	20 days	20 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Purity:91%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	Lysenin Protein, Eisenia fetida, Recombinant (His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 40.4 kDa and the accession number is O18423.
Species	Eisenia fetida
Expression System	E. coli
Tag	C-6xHis
Accession Number	O18423
Amino Acid	MSAKAAEGYEQIEVDVVAVWKEGYVYENRGSTSVDQKITITKGMKNVNSETRTVTATHSIGSTISTGDAFEIGSVEVSYSHSHEESQVSMTETEVYESKVIEHTITIPPTSKFTRWQLNADVGGADIEYMYLIDEVTPIGGTQSIPQVITSRAKIIVGRQIILGKTEIRIKHAERKEYMTVVSRKSWPAATLGHSKLFKFVLYEDWGGFRIKTLNTMYSGYEYAYSSDQGGIYFDQGTDNPKQRWAINKSLPLRHGDVVTFMNKYFTRSGLCYDDGPATNVYCLDKREDKWILEVVG
Construction	1-297 aa
Protein Purity	> 90% as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Synonyms	Lysenin, Invertebrate cytolysin pore, efL1, Beta-barrel pore-forming toxin (Beta-PFT)
Research Background	Pore-forming toxin that defensively acts against parasitic microorganisms by forming pores in sphingomyelin-containing membranes. Has hemolytic activity and is also cytotoxic to spermatozoa of some species of invertebrates and many species of vertebrates and to amphibian larvae, guinea pig polymorphonuclear leukocytes, chicken fibroblasts, normal spleen cells and various tumor cells. Is lethal for various species of reptiles, amphibian, birds and mammals. Induces smooth muscle contraction. It binds sphingomyelin and induces hemolysis in the same manner as lysenin-related protein 2, and is 10-fold more effective than lysenin-related protein 1.

Chemical Properties

Molecular Weight

40.4 kDa (predicted)

Storage & Solubility Information

Storage

Store at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

β-PFTβPFTβ-barrel pore-forming toxin (β-PFT)efL 1b-PFTbPFTBeta-PFTBetaPFTb-barrel pore-forming toxin (b-PFT)

Related Tags: Lysenin Protein, Eisenia fetida, Recombinant (His) chemical structure | Lysenin Protein, Eisenia fetida, Recombinant (His) molecular weight