1.Loaded LRP-6 Protein, Human, Recombinant (Avi & His), Biotinylated (Cat#TMPY-07087) on SA Biosensor, can bind DKK1 Protein, Human, Recombinant (His) (Cat#TMPY-00775) with an affinity constant of 90.6 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
2.Loaded LRP-6 Protein, Human, Recombinant (Avi & His), Biotinylated (Cat#TMPY-07087) on SA Biosensor, can bind RSPO1 Protein, Human, Recombinant (His) (Cat#TMPY-01147) with an affinity constant of 4.25 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested).
3.Loaded LRP-6 Protein, Human, Recombinant (Avi & His), Biotinylated (Cat#TMPY-07087) on SA Biosensor, can bind SOST/Sclerostin Protein, Human, Recombinant (His) (Cat#TMPY-00925) with an affinity constant of 39.5 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
4.Loaded LRP-6 Protein, Human, Recombinant (Avi & His), Biotinylated (Cat#TMPY-07087) on SA Biosensor, can bind DKK1 Protein, Human, Recombinant (hFc) (Cat#TMPY-05479) with an affinity constant of 54.3 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

LRP-6 Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 Cells. The accession number is O75581.

Human

HEK293 Cells

STHAG7,ADCAD2

A DNA sequence encoding the Human LRP6 (O75581) (Met1-Pro630) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

≥ 90% as determined by SDS-PAGE.

Please refer to the lot-specific COA.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Lipoprotein receptor-related protein 6 (LRP6) binds to Wnt ligands to transduce signal by stabilization of beta-catenin, which has been involved in the regulation of embryonic development and metabolism. Low density lipoprotein (LDL) receptor-related protein-6 (LRP6) is an important co-receptor of Wnt pathway, which plays a predominant role in the development and progression of colorectal cancer. This gene, termed LDL receptor-related protein 6 (LRP6), encodes a transmembrane protein that has 71% identity and is structurally similar to the protein encoded by LRP5, a proposed candidate gene for type 1 diabetes located on human chromosome 11q13. LRP6 maps to human chromosome 12p11-p13.