KIRREL1 Protein, Human, Recombinant (His)

Name: KIRREL1 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-00189
Rating: 4.5 (1 reviews)

Copy Product Info

Synonyms:

Catalog No. TMPY-00189 Copy Product Info

KIRREL1 Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q96J84-1.

Customer service consultation

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	7-10 days	7-10 days
10 µg	$109	7-10 days	7-10 days
20 µg	$172	7-10 days	7-10 days
50 µg	$343	7-10 days	7-10 days
100 µg	$682	7-10 days	7-10 days
200 µg	$987	7-10 days	7-10 days
500 µg	$2,200	7-10 days	7-10 days
1 mg	$4,500	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Resource Download

User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells. When cells are added to KIRREL coated plates (15 μg/mL, 100 μL/well), > 20% will adhere specifically after 90 minutes at 37 ℃
Description	KIRREL1 Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q96J84-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q96J84-1
Construction	A DNA sequence encoding the human KIRREL (NP_060710.3) (Met1-Ala499) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 95% as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Reconstitution	Please refer to the lot-specific COA.
Research Background	NEPH1 (KIRREL1) belongs to a family of three closely related transmembrane proteins of the Ig superfamily with a structure similar to that of nephrin. All three Neph proteins share a conserved podocin-binding motif; mutation of a centrally located tyrosine residue dramatically lowers the affinity of Neph1 for podocin. Neph1 triggers AP-1 activation similarly to nephrin but requires the presence of Tec family kinases for efficient transactivation. Neph1 consists of a signal peptide, five Ig-like C2-type domains with the middle domain overlapping with a PKD-like domain, an RGD sequence, a transmembrane domain, and a cytoplasmic tail, which is expressed in slit diaphragm domains of podocytes and vertebrate and invertebrate nervous systems. Neph1 is abundantly expressed in the kidney, specifically expressed in podocytes of kidney glomeruli, and plays a significant role in the normal development and function of the glomerular permeability. Neph1 interacts with nephrin in vitro and in vivo, and able to stimulate transcriptional activation in a model system, such as the activation of the transcription factor AP-1 via the stimulation of a MAPK module. Neph1 is crucial for the integrity of the slit diaphragm, as Neph1 gene knockout mice result in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death.

Chemical Properties

Molecular Weight	53.5 kDa (predicted)
Relative Density.	no data available

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.