IL-24 Protein, Human, Recombinant (His)

Name: IL-24 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-02087
Price: 42 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: ST16, MOB5, MDA7, interleukin 24, IL10B, FISP, C49A) Copy Product Info

Synonyms: ST16, MOB5, MDA7, interleukin 24, IL10B, FISP, C49A

Catalog No. TMPY-02087 Copy Product Info

Purity: 95.4%

IL-24 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 19.5 kDa and the accession number is A0A7R8C2Y5.

Pack Size	Price	USA Stock	Global Stock
5 µg	$42	-	In Stock
10 µg	$65	-	In Stock
20 µg	$98	-	In Stock
50 µg	$197	-	In Stock
100 µg	$333	7-10 days	7-10 days
200 µg	$570	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:95.4%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human IL20 Rα and human IL20 Rβ. The EC50 for this effect is typically 0.05-0.25 ng/mL.
Description	IL-24 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 19.5 kDa and the accession number is A0A7R8C2Y5.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q13007-2
Construction	A DNA sequence encoding the human IL24 isoform 3 (NP_001172085.1) (Met 1-Leu 207) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 53
Protein Purity	> 94 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	ST16, MOB5, MDA7, interleukin 24, IL10B, FISP, C49A
Research Background	Interleukin-24 (IL-24) also known as Melanoma differentiation-associated gene 7 protein (MDA-7) is a member of the IL10 family of cytokines. IL-24/MDA-7/IL24 can induce apoptosis selectively in various cancer cells. Overexpression of IL-24 leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Human IL-24/MDA-7/IL24 is secreted by activated peripheral blood mononuclear cells and is the ligand for two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2. Northern blot analysis revealed IL-24/MDA-7/IL24 expression in human tissues associated with the immune system such as the spleen, thymus, peripheral blood leukocytes, and normal melanocytes. IL-24/MDA-7/IL24 binding to either its endogenous receptors on human keratinocytes or to ectopically expressed receptors on baby hamster kidney cells leads to activation of the signal transducers and activators of transcription.

Chemical Properties

Molecular Weight

19.5 kDa (predicted); 35 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.