IL-8/CXCL8 Protein, Human, Recombinant (CHO)

Name: IL-8/CXCL8 Protein, Human, Recombinant (CHO)
Brand: TargetMol
SKU: TMPS-00092
Price: 85 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: neutrophil activating factor, NAP-1, NAF, monocyte-derived neutrophil chemotactic factor, MDNCF, IL8, CXCL8) Copy Product Info

Synonyms: neutrophil activating factor, NAP-1, NAF, monocyte-derived neutrophil chemotactic factor, MDNCF, IL8, CXCL8

Catalog No. TMPS-00092 Copy Product Info

IL-8/CXCL8 Protein, Human, Recombinant (CHO) is expressed in CHO Cells. The accession number is P10145.

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Pack Size	Price	USA Stock	Global Stock
5 µg	$85	-	In Stock
10 µg	$138	-	In Stock
20 µg	$197	-	In Stock
50 µg	$339	-	In Stock
100 µg	$552	-	In Stock
200 µg	$883	7-10 days	7-10 days
500 µg	$1,730	7-10 days	7-10 days
1 mg	$2,850	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:> 95% as analyzed by SDS-PAGE > 95% as analyzed by HPLC

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	ED 50 < 6.0 ng/ml, measured in a calcium flux assay using CHO/Gα15 cells transiently expressing CXCR1.
Description	IL-8/CXCL8 Protein, Human, Recombinant (CHO) is expressed in CHO Cells. The accession number is P10145.
Species	Human
Expression System	CHO Cells
Tag	Tag Free
Accession Number	P10145
Construction	Ala23-Ser99
Protein Purity	> 95% as determined by SDS-PAGE; > 95% as determined by HPLC
Endotoxin	< 0.2 EU/μg of protein as determined by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	neutrophil activating factor, NAP-1, NAF, monocyte-derived neutrophil chemotactic factor, MDNCF, IL8, CXCL8
Research Background	Interleukin-8 (IL-8), also known as CXCL8, GCP-1 and NAP-1, is one of the first discovered chemokines and belongs to the CXCL family, in which the first two conserved cysteines are separated by one residue. In vivo, IL-8 exists in two forms: a 77 a.a. protein produced by endothelial cells, and the more active 72 a.a. protein produced by monocytes. The receptors for IL-8 are the seven-helical G-protein coupled receptors CXCR1 and CXCR2, exclusively expressed on neutrophils. The functions of IL-8 are to induce rapid changes in cell morphology, activate integrins, and release the granule contents of neutrophils. Thus, IL-8 can enhance the antimicrobial actions of defense cells. It is secreted by monocytes, macrophages and endothelial cells. IL-8 signals through CXCR1 and CXCR2 to chemoattract neutrophils, basophils, and T cells. IL-8 is also a potent promoter of angiogenesis. Other functions of this protein, such as involvement in bronchiolitis pathogenesis, have also been reported.

Chemical Properties

Molecular Weight

~9 kDa (Reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.