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Synonyms: TCGF-2, T cell growth factor-2, MCGF-2, Mast-cell growth factor-2, interleukin 4, Il-4, IL4, BSF-1, BSF1, BSF 1, BCGF-1, BCDF, B cell stimulatory factor-1, B cell growth factor-1, B cell differentiation factor


| Bioactivity | Mouse IL-4 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-4, His Tag with an affinity constant of 21.13 pM as determined in SPR assay (Biacore S200). |
| Description | IL-4 Protein, Mouse, Recombinant (His) is expressed in HEK293 Cells. The accession number is P07750. |
| Species | Mouse |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | P07750 |
| Construction | His21-Ser140 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 0.1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | TCGF-2, T cell growth factor-2, MCGF-2, Mast-cell growth factor-2, interleukin 4, Il-4, IL4, BSF-1, BSF1, BSF 1, BCGF-1, BCDF, B cell stimulatory factor-1, B cell growth factor-1, B cell differentiation factor |
| Research Background |
| Molecular Weight | 15.17 kDa (Predicted); 20-30 kDa (Reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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