IL-3 Protein, Mouse, Recombinant (E. coli)

Name: IL-3 Protein, Mouse, Recombinant (E. coli)
Brand: TargetMol
SKU: TMPS-00165
Rating: 4.5 (1 reviews)

(Synonyms: P-cell stimulation factor, MULTI-CSF, MCGF, Mast cell growth factor, interleukin 3, IL-3, IL3, HCGF) Copy Product Info

Synonyms: P-cell stimulation factor, MULTI-CSF, MCGF, Mast cell growth factor, interleukin 3, IL-3, IL3, HCGF

Catalog No. TMPS-00165 Copy Product Info

IL-3 Protein, Mouse, Recombinant (E. coli) is expressed in E. coli. The accession number is P01586.

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Pack Size	Price	USA Stock	Global Stock
5 µg	$98	7-10 days	7-10 days
10 µg	$170	-	In Stock
20 µg	$295	7-10 days	7-10 days
50 µg	$622	-	In Stock
100 µg	$993	7-10 days	7-10 days
200 µg	$1,580	7-10 days	7-10 days
500 µg	$2,960	7-10 days	7-10 days
1 mg	$4,750	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:> 95% as analyzed by SDS-PAGE

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	ED 50 < 0.05 ng/ml, measured by a cell proliferation assay using M-NFS-60 cells, corresponding to a specific activity of > 2.0 × 10 7 units/mg.
Description	IL-3 Protein, Mouse, Recombinant (E. coli) is expressed in E. coli. The accession number is P01586.
Species	Mouse
Expression System	E. coli
Tag	Tag Free
Accession Number	P01586
Construction	Asp33-Cys166
Protein Purity	> 95% as determined by SDS-PAGE
Endotoxin	< 0.2 EU/μg of protein as determined by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	P-cell stimulation factor, MULTI-CSF, MCGF, Mast cell growth factor, interleukin 3, IL-3, IL3, HCGF
Research Background	Interleukin-3 (IL-3) is a pleiotropic cytokine belonging to the interleukin family. IL-3 shares similarities with Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) and IL-5: they all have a four-helix bundle structure, are located on the same chromosomes in both human and mouse, are produced by activated T cells, and share receptors. The IL-3/IL-5/GM-CSF receptor family members are all heterodimeric, composed of a receptor-specific α chain and a common β chain. IL-3 is also called multi-colony stimulating factor since it stimulates the development and colony formation of multiple lineages of hematopoietic cells by activating intracellular pathways such as Ras-Raf-ERK and JAK/STAT. IL-3 inhibits apoptosis and promotes cell survival by targeting the anti-apoptotic bcl-2 gene family.

Chemical Properties

Molecular Weight

~15.2 kDa (Non-reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.