Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 ng/mL. (QC Test)
Immobilized Human IL-13, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 3.6 ng/ml determined by ELISA. (QC Test)
Loaded Human IL-13Ra2, His Tag on Anti-His-Biosensor can bind Human IL-13, No Tag with an affinity constant of 0.63 nM as determined in BLI assay (Gator® Prime).

IL-13 Protein, Human, Recombinant (E. coli) is expressed in E. coli. The accession number is AAK53823.

Human

E. coli

P600,interleukin 13,IL-13,ALRH

Gly21-Asn132

> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).