ED 50 < 10.0 pg/ml, measured by a cell proliferation assay using mouse D10S cells, corresponding to a specific activity of > 1.0 × 10^8 units/mg.

IL-1 beta/IL-1F2 Protein, Rat, Recombinant is expressed in E. coli. The accession number is Q63264.

Rat

E. coli

interleukin 1, β,IL-1 β

Val117-Ser268

> 95% as determined by SDS-PAGE > 95% as determined by HPLC

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Interleukin-1 Beta (IL-1 Beta) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta bind to the same receptor and have similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.