IFN-alpha 2b/IFNA2 Protein, Human, Recombinant

At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end.

Pack Size	Availability	Price/USD
10 μg	In stock	$ 38.00
20 μg	5 days	$ 60.00
50 μg	5 days	$ 110.00
100 μg	5 days	$ 231.00

Pack Size

Availability

Price/USD

Quantity

10 μg

In stock

$ 38.00

20 μg

5 days

$ 60.00

50 μg

5 days

$ 110.00

100 μg

5 days

$ 231.00

Biological Information

1. Loaded Human IFNAR2-Fc on Protein A Biosensor, can bind Human IFN alpha2b with an affinity constant of 2.98 nM as determined in BLI assay. (Regularly tested) 2. Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV) The ED50 for this effect is 5 ng/mL. (Regularly tested)

Description

Species	Human
Expression System	E. coli
Tag	None
Accession Number	P01563
Synonyms	IFNA2, Interferon α-2, Interferon α-A, Interferon Alpha-2, IFN-α-2, LeIF A, Interferon Alpha-A, IFN-Alpha-2, IFN α2b
Amino Acid	Cys24-Glu188
Construction	Recombinant Human Interferon Alpha-2b is produced by our E.coli expression system and the target gene encoding Cys24-Glu188 is expressed.
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
Molecular Weight	17 KDa, reducing conditions

Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stability & Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Research Background	At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end.

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IFN-alpha 2b/IFNA2 Protein, Human, Recombinant

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