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Hygromycin-B 4-O-kinase Protein, E. coli, Recombinant (His)

(Synonyms: Hygromycin-B kinase, Hygromycin-B 4-O-kinase, Hygromycin B phosphotransferase, hph, APH(4)) Copy Product Info

Synonyms: Hygromycin-B kinase, Hygromycin-B 4-O-kinase, Hygromycin B phosphotransferase, hph, APH(4)

Catalog No. TMPH-00639 Copy Product Info
The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation. Only phosphorylates hygromycin and closely related compounds such as demethyl analogs and destomycin.
Hygromycin-B 4-O-kinase Protein, E. coli, Recombinant (His)
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Pack SizePriceUSA StockGlobal StockQuantity
5 µg$12920 days20 days
10 µg$21620 days20 days
20 µg$36020 days20 days
50 µg$54320 days20 days
100 µg$74520 days20 days
200 µg$1,07020 days20 days
500 µg$1,73020 days20 days
1 mg$2,53020 days20 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation. Only phosphorylates hygromycin and closely related compounds such as demethyl analogs and destomycin.
Species
E. coli
Expression System
E. coli
TagN-6xHis
Accession NumberP00557
Amino AcidMKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
Construction1-341 aa
Protein Purity
> 85% as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationTris-based buffer, 50% glycerol
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsHygromycin-B kinase, Hygromycin-B 4-O-kinase, Hygromycin B phosphotransferase, hph, APH(4)
Research Background
The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation. Only phosphorylates hygromycin and closely related compounds such as demethyl analogs and destomycin.
Chemical Properties
Molecular Weight42.0 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: Hygromycin-B 4-O-kinase Protein, E. coli, Recombinant (His) chemical structure | Hygromycin-B 4-O-kinase Protein, E. coli, Recombinant (His) molecular weight