GSN Protein, Human, Recombinant (mFc)

Gelsolin is a calcium-activated actin filament severing and capping protein found in many cell types and as a secreted form in the plasma of vertebrates. Gelsolin is composed of six of these domains, termed G1-6. Some reaseraches show that gelsolin can act as a transcriptional cofactor in signal transduction and its own expression and function can be influenced by epigenetic changes. The difference in the expression levels of cytoplasmic and plasma gelsolin suggests that these two different forms of gelsolin may play different roles after cardiac injury. Plasma gelsolin, as an important component of the EASS, may have evolved to rapidly clear actin filaments from the circulation that are released by injured or dead cells.

Pack Size	Availability	Price/USD
10 μg	5 days	$ 160.00
50 μg	5 days	$ 479.00
500 μg	5 days	$ 2,420.00
1 mg	5 days	$ 3,630.00

Pack Size

Availability

Price/USD

Quantity

10 μg

5 days

$ 160.00

50 μg

5 days

$ 479.00

500 μg

5 days

$ 2,420.00

1 mg

5 days

$ 3,630.00

Description

Species	Human
Expression System	Human Cells
Tag	C-mFc
Accession Number	P06396
Synonyms	ADF, Actin-depolymerizing factor, Brevin, Gelsolin, GSN, AGEL
Amino Acid	Ala28-Ala782
Construction	Recombinant Human Gelsolin is produced by our Mammalian expression system and the target gene encoding Ala28-Ala782 is expressed with a mouse IgG1 Fc tag at the C-terminus.
Protein Purity	Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight	115-125 KDa, reducing conditions

Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM EDTA, 1mM DTT, 5% Trehalose, 0.1% Triton X-100, pH 8.0.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stability & Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Research Background	Gelsolin is a calcium-activated actin filament severing and capping protein found in many cell types and as a secreted form in the plasma of vertebrates. Gelsolin is composed of six of these domains, termed G1-6. Some reaseraches show that gelsolin can act as a transcriptional cofactor in signal transduction and its own expression and function can be influenced by epigenetic changes. The difference in the expression levels of cytoplasmic and plasma gelsolin suggests that these two different forms of gelsolin may play different roles after cardiac injury. Plasma gelsolin, as an important component of the EASS, may have evolved to rapidly clear actin filaments from the circulation that are released by injured or dead cells.

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GSN Protein, Human, Recombinant (mFc)

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