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GAPDH Protein, Mouse, Recombinant (His)

(Synonyms: glyceraldehyde-3-phosphate dehydrogenase, Gapd) Copy Product Info

Synonyms: glyceraldehyde-3-phosphate dehydrogenase, Gapd

Catalog No. TMPY-00506 Copy Product Info
GAPDH Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 37.9 kDa and the accession number is P16858.
Pack SizePriceUSA StockGlobal StockQuantity
5 µg$79-In Stock
10 µg$129-In Stock
20 µg$208-In Stock
50 µg$419-In Stock
100 µg$8127-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:> 85 % as determined by SDS-PAGE
Appearance:Lyophilized powder
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Product Introduction

Bioactivity
Bioactivity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
GAPDH Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 37.9 kDa and the accession number is P16858.
Species
Mouse
Expression System
E. coli
TagN-His
Accession NumberP16858
ConstructionA DNA sequence encoding the mouse GAPDH (P16858) (Met1-Glu333) was expressed with a polyhistidine tag at the N-terminus. Predicted N terminal: His
Protein Purity
> 85 % as determined by SDS-PAGE
EndotoxinPlease contact us for more information.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 200 mM Arg, 10% gLycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with 50 mM Tris, 200 mM Arg to 0.63 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonymsglyceraldehyde-3-phosphate dehydrogenase, Gapd
Research Background
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.
Chemical Properties
Molecular Weight37.9 kDa (predicted); 38 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: GAPDH Protein, Mouse, Recombinant (His) chemical structure | GAPDH Protein, Mouse, Recombinant (His) molecular weight