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Synonyms: glyceraldehyde-3-phosphate dehydrogenase, Gapd

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $79 | - | In Stock | |
| 10 µg | $129 | - | In Stock | |
| 20 µg | $208 | - | In Stock | |
| 50 µg | $419 | - | In Stock | |
| 100 µg | $812 | 7-10 days | 7-10 days |
| Bioactivity | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| Description | GAPDH Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 37.9 kDa and the accession number is P16858. |
| Species | Mouse |
| Expression System | E. coli |
| Tag | N-His |
| Accession Number | P16858 |
| Construction | A DNA sequence encoding the mouse GAPDH (P16858) (Met1-Glu333) was expressed with a polyhistidine tag at the N-terminus. Predicted N terminal: His |
| Protein Purity | > 85 % as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 200 mM Arg, 10% gLycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with 50 mM Tris, 200 mM Arg to 0.63 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Synonyms | glyceraldehyde-3-phosphate dehydrogenase, Gapd |
| Research Background | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions. |
| Molecular Weight | 37.9 kDa (predicted); 38 kDa (reducing condition, due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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