Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is 5-20 ng/mL.

FGF-8b Protein, Human, Recombinant V2 is expressed in E. coli. The accession number is P55075-3.

Human

E. coli

NEWENTRY,KAL6,HH6,HBGF-8,fibroblast growth factor 8 (androgen-induced),FGF-8,AIGF

Gln23-Arg215

> 95% as determined by Tris-Bis PAGE

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Two of the four human FGF8 splice isoforms, FGF8a and FGF8b, are expressed in the mid-hindbrain region during development. Although the only difference between these isoforms is the presence of an additional 11 amino acids at the N terminus of FGF8b, these isoforms possess remarkably different abilities to pattern the midbrain and anterior hindbrain.