ED 50 < 1.0 ng/ml, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 1.0 × 10 6 units/mg.

FGF-2 Protein, Bovine, Recombinant is expressed in E. coli. The accession number is P03969.

Bovine

E. coli

Prostatropin,HBGF-2,Fibroblast Growth Factor-basic,FGFB,FGF-2,BFGF,bFGF

Pro10-Ser155

> 95% as determined by SDS-PAGE

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in PBS up to 100 μg/ml.（It is recommended to increase the concentration of NaCl in PBS to 300 mM）

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.