EphB1 Protein, Mouse, Recombinant (GST)

Name: EphB1 Protein, Mouse, Recombinant (GST)
Brand: TargetMol
SKU: TMPC-00257
Rating: 4.5 (1 reviews)

(Synonyms: Ephrin type-B receptor 1, Ephb1) Copy Product Info

Synonyms: Ephrin type-B receptor 1, Ephb1

Catalog No. TMPC-00257 Copy Product Info

EphB1 Protein, Mouse, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is Q8CBF3.

Pack Size	Price	USA Stock	Global Stock	Quantity
10 µg	$1,390	35 days	35 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	The specific activity of EPHB1 was determined to be 82 nmol/min/mg by ADP-Glo kinase assay.
Description	EphB1 Protein, Mouse, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is Q8CBF3.
Species	Mouse
Expression System	Baculovirus-Insect Cells
Tag	N-GST
Accession Number	Q8CBF3
Construction	Recombinant mouse EPHB1 (591-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Protein Purity	>90% as determined by SDS-PAGE.
Formulation	Supplied as sterile 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Synonyms	Ephrin type-B receptor 1, Ephb1
Research Background	Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Chemical Properties

Molecular Weight

~62 kDa

Storage & Solubility Information

Shipping	Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.