EGF Protein, Canine, Recombinant

Name: EGF Protein, Canine, Recombinant
Brand: TargetMol
SKU: TMPY-04884
Rating: 4.5 (1 reviews)

(Synonyms: epidermal growth factor) Copy Product Info

Synonyms: epidermal growth factor

Catalog No. TMPY-04884 Copy Product Info

EGF Protein, Canine, Recombinant is expressed in yeast. The predicted molecular weight is 6.2 kDa and the accession number is Q9BEA0.

Pack Size	Price	USA Stock	Global Stock
5 µg	$34	7-10 days	7-10 days
10 µg	$49	7-10 days	7-10 days
20 µg	$79	7-10 days	7-10 days
50 µg	$155	7-10 days	7-10 days
100 µg	$296	7-10 days	7-10 days
200 µg	$425	7-10 days	7-10 days
500 µg	$838	7-10 days	7-10 days
1 mg	$1,620	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured in a cell proliferation assay using Balb 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1-0.6 ng/mL.
Description	EGF Protein, Canine, Recombinant is expressed in yeast. The predicted molecular weight is 6.2 kDa and the accession number is Q9BEA0.
Species	Canine
Expression System	P. pastoris (Yeast)
Tag	Tag Free
Accession Number	Q9BEA0
Construction	A DNA sequence encoding the canine EGF (NP_001003094.1) (Asn973-Arg1024) was expressed. Predicted N terminal: Asn 973
Protein Purity	> 95 % as determined by SDS-PAGE.
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	epidermal growth factor
Research Background	EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.

Chemical Properties

Molecular Weight

6.2 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.